Evaluation of Physicochemical Properties of Nano-Sized Hydroxyapatite Particles Synthesized from Cowbones

Abstract

 Pure and white Hydroxyapatite Ca₁₀(PO₄)₆ (OH)₂ powder was synthesized from animal bone (Cowbone) using thermal decomposition method. The physicochemical properties of the synthesized hydroxyapatite were evaluated using X-ray diffraction analysis and Fourier Transform Infrared spectroscopy. The X-ray diffraction results revealed crystalline phase of hydroxyapatite while Scanning Electron Microscope analysis revealed the microscopic structure and nano size of the hydroxyapatite particle. Fourier Transform Infrared spectroscopy was used to identify functional groups in the material and did not indicate any organic components of the bone. Thermo gravimetric analysis confirmed the thermal stability of the hydroxyapatite. The in-vivo cytotoxicity test using Wistar Albino Rats did not indicate any toxic effects of the material at acute level. The research has not only shown economic potentiality of producing nano hydroxyapatite from this waste but offers a means of impacting positively on the environment.