GC-MS, Phytochemical and Antimicrobial Analysis of the Leaf of Newbouldia Laevis (P.Beav)

Abstract

The leaf of Newbouldia laevis were analyzed for its phytochemical and anti-microbial properties with the aid of; Shimazu Japan GC model 5890-11, GC-MS QP 2010 PLUS Shimazu Japan and Jenway digital Spectrophtometer model 6303. The GC-MS analysis of this sample gave the following compounds; 2- phenoxy ethyl beta -2-phenoxy ethyl-3-phenyl propanoate (1.41%), benzene-(1-methyl nonadecyl) (1.41 %), 6-phenyl undecane (1.06%), Eicosyne (2.47 %), hexadecanoic acid methyl ester (13.73 %), n-hexadecanoic acid (9.16%), z-octadecanoic acid (22.81 %) ,9-octadecanoic acid (22.81 %) phytol (23.51 %) Oleic acid (10.56 %), octadecanoic acid. (5.97 %), squalene (7.75 %). The phytochemical composition of the sample showed that the alkaloid composition was 0.48 %, with corresponding values of 0.86.%, 0.66 %, 0.024 % and 0.86 ppm for flavonoids ,saponins, phenols and tannins respectively The extracts showed marked inhibition of the growth of seven selected pathogens; Pseudomonas aeruginosa 12 mm, Staphylococcus aureus 13 mm, Escherichia coli 15 mm, Salmonella typhi  17 mm, Candida albican 11 mms, Aspergillus niger 14 mm and Penicillium12mm with minimum inhibitory concentrations of 6.5  mg/cm³, 12.5  mg/cm³ ,12.5  mg/cm³, 6.5 mg/cm³,12.5. mg/cm³, 12.5 mg/cm³ and 6.5 mg/cm³   respectively