Antibacterial Potential of Phytochemicals against Urinary Tract Infectious Bacteria

Abstract

Urinary Tract Infections represent one of the most common diseases encountered in medical practices. The clinical significance of the disease is due to its high mortality rate, malignant and chronic hypertensions and chronic renal failures. The present study involves the isolation of bacterial strains by catheterization and identification based on their morphological characterization and biochemical analysis. The disc diffusion assay was performed for a comparative analysis to identify the most suitable plant extract and its active compound that could be used in curing ailments of Urinary Tract Infections. The plant extracts of Eupatorium odoratum L.,Azadirachta indica L., Ocimum sanctum L. Coleus aromaticusL., Aloe barbadensis L., Rotula aquatica L, were analyzed for their antibacterial activity against the isolates. Further, the active compounds, lignins and proteins were isolated from the effective extracts of Eupatorium odoratum L. and Rotula aquatica L. and tested for their antibacterial activity which could be used in the formulation of various medicines. Four different bacterial isolates obtained were identified asE. coli,Klebsiella pneumoniae,Pseudomonas aeruginosa and Staphylococcus aureus. The most effective plant extracts were that of Eupatorium odoratum L. and Rotula aquatica L. The protein extract of Eupatorium odoratum L. and Rotula aquatia L.proved to be effective constituent as antibacterial agent.The excessive use of antibiotics paves way for secondary ailments and resistance between different bacterial species, hence there is a rapid replacement of chemical antibiotics with natural remedies which is highly demanded for their non toxicity and lesser side effects and better cure.