Determination of Antibiotic Resistance of Salmonella and Molecular Confirmation of Drug Resistance Salmonella Species Isolated From Raw Chicken and Quail Eggs from Selected Farms in Jos, Nigeria

Abstract

Determination of antibiotic resistance of Salmonella and molecular confirmation of drug resistance Salmonella species isolated from raw chicken and quail eggs from selected poultry farms in Jos Local Government Area was carried out. A total of 360 egg samples were randomly collected, one hundred and eighty (180) each from quail and chicken respectively from three Local Government Areas namely; Jos north. Jos south and Jos east were sampled. The samples were examined for the presence of Salmonella species using standard microbiological and biochemical techniques. They were tested for antimicrobial susceptibility by disc diffusion method. Results showed that out of the 360 samples, only 3(1.7%) from chicken egg shells were positive for Salmonella. Positive result for Salmonella was not obtained from quail egg shells nor contents. Isolates from results were further subjected to susceptibility testing to eleven (11) commonly used antibiotics. Three (3) were 100% resistant to Streptomycin, Erthromyc in and Oxacillin. The isolates were also 67% resistant to Ampicillin, Ciprofloxacin and Chloramphenicol. All the three (3) isolates were successfully amplified using specific primers for PCR method. The results showed presence of Salmonella species in only chicken egg shells. Poultry eggs should be properly washed before consuming it raw so as to reduce infections by this organism.